Optimization

Proprietary workflows and AI/ML technologies for antibody lead optimization

Dial in key binding and developability parameters for your therapeutic antibody.

Develop therapeutics optimized for affinity, specificity and drug-like properties 

Our experienced teams leverage the latest in silico tools and mAbForge, our proprietary high-throughput characterization platform, to enhance your antibody’s function while removing liabilities, helping you develop safer, more efficacious drugs.  

Enhanced Function

Fine tune affinity, cross-reactivity, or Fc effector function to improve the therapeutic potency, specificity, and PK/PD of your leads.

Reduced Risk

Eliminate liabilities like polyreactivity, poor stability, or predicted immunogenicity to minimize costly setbacks and accelerate the development path for your antibody-based drugs.

Integrated Workflows

Combine antibody optimization with binder discovery, bispecific engineering, functional testing, and robust analytical characterization to streamline end-to-end development of your drug candidates.

Offers

Optimizing your antibody to meet your target product profile and therapeutic goals​

AI/ML powered in silico design

Our world-class computational team uses the latest AI/ML tools to predict optimal variants based on deep analysis of antibody sequence and structural data.  For each optimization project, they design tailored antibody diversity in panels of hundreds of individual clones or libraries with billions of variants.  ML-trained models are used to predict liabilities like hydrophobicity and polyreactivity, allowing us to remove problematic variants prior to testing.

Rapid build-test cycles with mAbForge™ to find your lead faster​

mAbForge, Alloy’s proprietary high-throughput expression and characterization platform that allows us to go from in silico sequences to hundreds of purified antibodies with affinity, specificity, and cell binding data in as little as 3 weeks.  

Panels of computationally designed variants are rapidly optimized via iterative design-build-test cycles  Typically 2-3 rounds of in silico design and mAbForge testing is sufficient to hit your desired antibody properties.  

For more complex projects, we can also design libraries with billions of antibody variants and use our phage and yeast display platforms to enrich for optimal clones.

Optimize multiple parameters to improve efficacy and safety​

With Alloy’s iterative design, build, and test capabilities, we can simultaneously optimize multiple antibody parameters including affinity, specificity, and developability to ensure that your lead candidates are meeting your target product profile.

With our rapid design-build-test cycles, we can quickly enhance affinities to improve potency or detune affinities to improve safety.  

Eliminate off-target binding for improved safety or enhance cross-reactivity to different species for a streamlined translational path or to related antigens for broader activity.

Develop antibodies with reduced or enhanced affinity at lower pH to create drugs with altered function in the endosome or tumor.

Starting with a murine, camelid, or rabbit antibody from another source?  Rapidly humanize binders leveraging our  in silico design and high-throughput production platforms.  

We utilize computational tools to identify potentially immunogenic epitopes and design alternative, lower risk variants while maintaining affinity and function.

Our design, engineering, and analytical capabilities deliver enhanced stability, reduced polyreactivity or hydrophobicity, and eliminate sequence liabilities to ensure your leads are manufacturable and safe.

Pair your binding arms with an Fc domain engineered for desired pharmacokinetics and effector function.

Partner and optimize with us

Launch your optimization efforts with a bespoke project that delivers on your therapeutic application goals

Contact us to discuss your optimization needs with our team

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Explore Alloy’s additional offerings to help achieve your therapeutic goals

Learn more about our full suite of services and offerings designed to accelerate your path from target to IND.

Monoclonal antibody discovery

Accelerated antibody discovery tailored to your target product profile.

Bispecific antibody discovery

Comprehensive end-to-end discovery workflow including customized engineering and rank-ordered candidates for your target.